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Cells were incubated with the agonist for 15 min (except in the case of KRAS assays, where the incubation time was 60 min), and bioluminescence resonance energy transfer (BRET) measurements were taken using a Victor X4 plate reader (PerkinElmer) immediately after the addition of coelenterazine h (final concentration: 1 M)
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PubMed laboratory research record [PMID: 15562830] PubMed record related to this compound for laboratory literature review
Weight management demand contributes to nearly 52% of therapy usage growth, while cardiovascular benefits influence 49% of prescribing decisions, making these drugs essential in modern metabolic disorder treatment protocols
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